Thursday, February 12, 2009
complaints for the ether
I'm trying to go back to my sequencing data to verify the quality of the contigs but when I open the raw quality file and search out the four contigs of interest they are there, but much much shorter than the contigs I'm dealing with now. So either the numbers don't correspond like I assumed or I have amalgamated multiple contigs into one long one, and they mis-leadingly maintained the name of a single contig. If option 2, I can't remember exactly my steps and I don't think I wrote them down. I don't know how to retrace my steps, and so I am screwed in terms of determining quality scores for my contigs of interest. F&ck.
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